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KMID : 0829320150180010027
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Korean Journal of Clinical Microbiology
2015 Volume.18 No. 1 p.27 ~ p.32
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Evaluation of the Performance of a New Chromatographic Assay BD Veritor System for Rapid Detection of Influenza A & B
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Park Shol-Hui
Lee Mi-Ae
Chung Hae-Sun
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Abstract
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Background: For early diagnosis and treatment of influenza, rapid influenza diagnostic tests are commonly used. We evaluated the performance of the BD Veritor System for Rapid Detection of Flu A＋B(BD Veritor System; BD Diagnostics, USA) compared to multiplex real-time RT-PCR.
Methods: A total of 3,213 nasal and nasopharyngeal swab specimens in transport media from patients with influenza-like symptoms were tested with the BD Veritor System from December 2013 to April 2014.The sensitivity and specificity of 127 specimens were determined simultaneously using multiplex real-timeRT- PCR with the AdvanSure RV real-time PCR(AdvanSure PCR; LG Life Sciences, Korea).
Results: Influenza viruses were detected in 41.3%(1,327/3,213) of all specimens tested using the BD Veritor System. Of the 127 specimens, 27 influenza A and 17 influenza B viruses were identified by the AvanSure PCR. The sensitivity and specificity of the BD Veritor System relative to the AdvanSure PCR was 85.2% and 99.0% for influenza A, and 58.8% and 99.1% for influenza B. Of the 190 specimens that tested negative using the BD Veritor System, theAdvanSure PCR detected influenza A and influenza B in 19 and 13 specimens, respectively. The mean threshold cycle (Ct) values of the antigen positive specimens were lower than those of the antigen negative specimens.
Conclusion: The BD Veritor System showed excellent specificity for both influenza types and good sensitivity for influenza A. However, the system was less sensitive for influenza B compared to multiplex real-time RT-PCR. For accurate diagnosis of false negative specimens, a molecular diagnostic test should be performed. The BD Veritor system could be a useful tool for screening and early diagnosis of influenza.
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KEYWORD
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Influenza, Rapid influenza diagnostic test, Real-time RT-PCR, Sensitivity
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